Publications

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Publications in peer reviewed journals

2 Publications found
  • Insecticidal Toxicity of Involves the Novel Enterotoxin YacT.

    Springer K, Sänger PA, Moritz C, Felsl A, Rattei T, Fuchs TM
    2018 - Front Cell Infect Microbiol, 392

    Abstract: 

    The genus comprises 19 species of which three are known as human and animal pathogens. Some species display toxicity toward invertebrates using the so-called toxin complex (TC) and/or determinants that are not yet known. Recent studies showed a remarkable variability of insecticidal activities when representatives of different species (spp.) were subcutaneously injected into the greater wax moth, . Here, we demonstrate that and are highly toxic to this insect. A member of phylogroup 1B killed larvae with injection doses of approximately 38 cells only, thus resembling the insecticidal activity of . The pathogenicity spp. displays toward the larvae was higher at 15°C than at 30°C and independent of the TC. However, upon subtraction of all genes of the low-pathogenic strain W22703 from the genomes of and , we identified a set of genes that may be responsible for the toxicity of these two species. Indeed, a mutant of lacking , a gene that encodes a protein similar to the heat-stable cytotonic enterotoxin (Ast) of , exhibited a reduced pathogenicity toward larvae and altered the morphology of hemocytes. The data suggests that the repertoire of virulence determinants present in environmental species remains to be elucidated.

  • A promiscuous beta-glucosidase is involved in benzoxazinoid deglycosylation in Lamium galeobdolon.

    Hannemann L, Lucaciu CR, Sharma S, Rattei T, Mayer KFX, Gierl A, Frey M
    2018 - Phytochemistry, 224-233

    Abstract: 

    In the plant kingdom beta-glucosidases (BGLUs) of the glycosidase hydrolase family 1 have essential function in primary metabolism and are particularly employed in secondary metabolism. They are essential for activation in two-component defence systems based on stabilisation of reactive compounds by glycosylation. Based on de novo assembly we isolated and functionally characterised BGLUs expressed in leaves of Lamium galeobdolon (LgGLUs). LgGLU1 could be assigned to hydrolysis of the benzoxazinoid GDIBOA (2,4-dihydroxy-1,4-benzoxazin-3-one glucoside). Within the Lamiaceae L. galeobdolon is distinguished by the presence GDIBOA in addition to the more common iridoid harpagide. Although LgGLU1 proved to be promiscuous with respect to accepted substrates, harpagide hydrolysis was not detected. Benzoxazinoids are characteristic defence compounds of the Poales but are also found in some unrelated dicots. The benzoxazinoid specific BGLUs have recently been identified for the grasses maize, wheat, rye and the Ranunculaceae Consolida orientalis. All enzymes share a general substrate ambiguity but differ in detailed substrate pattern. The isolation of the second dicot GDIBOA glucosidase LgGLU1 allowed it to analyse the phylogenetic relation of the distinct BGLUs also within dicots. The data revealed long periods of independent sequence evolution before speciation.

Book chapters and other publications

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